hnpc line Search Results


hnpc line 15167  (Lonza)
90
Lonza hnpc line 15167
RNAi plays antiviral roles in <t>ZIKV-infected</t> hNPCs. a hNPCs in which Dicer1 or Ago2 were knocked down (K.D.) via the corresponding siRNAs (siRNA-1 and siRNA-2, 25 nM) or treated with a specific control siRNA (N.C., 25 nM) were infected with ZIKV at an MOI of 0.02. Forty-eight hours later, the amounts of ZIKV genomic RNA in each culture supernatant was determined by RT-qPCR. <t>b</t> <t>hNPC</t> or Dicer KO cell lines were infected with ZIKV at an MOI of 2. Northern blot assay of vsRNAs in cells at 48 hpi. miR-92a and U6 were used as internal references. c hNPCs that stably express Nov B2 or mB2 were infected with ZIKV at an MOI of 0.02. The amounts of ZIKV genomic RNA in the culture supernatants were determined by RT-qPCR at 48 hpi. d hNPCs treated with N.C. siRNA or ZIKV-specific siRNAs (10 μM) were infected with ZIKV at an MOI of 0.02 and analyzed using a ZIKV-specific RT-qPCR assay. All data are expressed as the means ± SD of three independent experiments. Statistical significance was determined by a Student’s t-test using GraphPad Prism. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
Hnpc Line 15167, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hnpc+line/pmc06461993-232-14-23?v=Lonza
Average 90 stars, based on 1 article reviews
hnpc line 15167 - by Bioz Stars, 2026-08
90/100 stars
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human neural progenitor cell line hnpc 15167  (Lonza)
90
Lonza human neural progenitor cell line hnpc 15167
RNAi plays antiviral roles in <t>ZIKV-infected</t> hNPCs. a hNPCs in which Dicer1 or Ago2 were knocked down (K.D.) via the corresponding siRNAs (siRNA-1 and siRNA-2, 25 nM) or treated with a specific control siRNA (N.C., 25 nM) were infected with ZIKV at an MOI of 0.02. Forty-eight hours later, the amounts of ZIKV genomic RNA in each culture supernatant was determined by RT-qPCR. <t>b</t> <t>hNPC</t> or Dicer KO cell lines were infected with ZIKV at an MOI of 2. Northern blot assay of vsRNAs in cells at 48 hpi. miR-92a and U6 were used as internal references. c hNPCs that stably express Nov B2 or mB2 were infected with ZIKV at an MOI of 0.02. The amounts of ZIKV genomic RNA in the culture supernatants were determined by RT-qPCR at 48 hpi. d hNPCs treated with N.C. siRNA or ZIKV-specific siRNAs (10 μM) were infected with ZIKV at an MOI of 0.02 and analyzed using a ZIKV-specific RT-qPCR assay. All data are expressed as the means ± SD of three independent experiments. Statistical significance was determined by a Student’s t-test using GraphPad Prism. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
Human Neural Progenitor Cell Line Hnpc 15167, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hnpc+line/pmc10603150-235-0-11?v=Lonza
Average 90 stars, based on 1 article reviews
human neural progenitor cell line hnpc 15167 - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
human np cell line hnpc  (ScienCell)
90
ScienCell human np cell line hnpc
RNAi plays antiviral roles in <t>ZIKV-infected</t> hNPCs. a hNPCs in which Dicer1 or Ago2 were knocked down (K.D.) via the corresponding siRNAs (siRNA-1 and siRNA-2, 25 nM) or treated with a specific control siRNA (N.C., 25 nM) were infected with ZIKV at an MOI of 0.02. Forty-eight hours later, the amounts of ZIKV genomic RNA in each culture supernatant was determined by RT-qPCR. <t>b</t> <t>hNPC</t> or Dicer KO cell lines were infected with ZIKV at an MOI of 2. Northern blot assay of vsRNAs in cells at 48 hpi. miR-92a and U6 were used as internal references. c hNPCs that stably express Nov B2 or mB2 were infected with ZIKV at an MOI of 0.02. The amounts of ZIKV genomic RNA in the culture supernatants were determined by RT-qPCR at 48 hpi. d hNPCs treated with N.C. siRNA or ZIKV-specific siRNAs (10 μM) were infected with ZIKV at an MOI of 0.02 and analyzed using a ZIKV-specific RT-qPCR assay. All data are expressed as the means ± SD of three independent experiments. Statistical significance was determined by a Student’s t-test using GraphPad Prism. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001
Human Np Cell Line Hnpc, supplied by ScienCell, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hnpc+line/pm34239363-70-1-9?v=ScienCell
Average 90 stars, based on 1 article reviews
human np cell line hnpc - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier

Image Search Results


RNAi plays antiviral roles in ZIKV-infected hNPCs. a hNPCs in which Dicer1 or Ago2 were knocked down (K.D.) via the corresponding siRNAs (siRNA-1 and siRNA-2, 25 nM) or treated with a specific control siRNA (N.C., 25 nM) were infected with ZIKV at an MOI of 0.02. Forty-eight hours later, the amounts of ZIKV genomic RNA in each culture supernatant was determined by RT-qPCR. b hNPC or Dicer KO cell lines were infected with ZIKV at an MOI of 2. Northern blot assay of vsRNAs in cells at 48 hpi. miR-92a and U6 were used as internal references. c hNPCs that stably express Nov B2 or mB2 were infected with ZIKV at an MOI of 0.02. The amounts of ZIKV genomic RNA in the culture supernatants were determined by RT-qPCR at 48 hpi. d hNPCs treated with N.C. siRNA or ZIKV-specific siRNAs (10 μM) were infected with ZIKV at an MOI of 0.02 and analyzed using a ZIKV-specific RT-qPCR assay. All data are expressed as the means ± SD of three independent experiments. Statistical significance was determined by a Student’s t-test using GraphPad Prism. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Journal: Cell Research

Article Title: Zika virus infection induces RNAi-mediated antiviral immunity in human neural progenitors and brain organoids

doi: 10.1038/s41422-019-0152-9

Figure Lengend Snippet: RNAi plays antiviral roles in ZIKV-infected hNPCs. a hNPCs in which Dicer1 or Ago2 were knocked down (K.D.) via the corresponding siRNAs (siRNA-1 and siRNA-2, 25 nM) or treated with a specific control siRNA (N.C., 25 nM) were infected with ZIKV at an MOI of 0.02. Forty-eight hours later, the amounts of ZIKV genomic RNA in each culture supernatant was determined by RT-qPCR. b hNPC or Dicer KO cell lines were infected with ZIKV at an MOI of 2. Northern blot assay of vsRNAs in cells at 48 hpi. miR-92a and U6 were used as internal references. c hNPCs that stably express Nov B2 or mB2 were infected with ZIKV at an MOI of 0.02. The amounts of ZIKV genomic RNA in the culture supernatants were determined by RT-qPCR at 48 hpi. d hNPCs treated with N.C. siRNA or ZIKV-specific siRNAs (10 μM) were infected with ZIKV at an MOI of 0.02 and analyzed using a ZIKV-specific RT-qPCR assay. All data are expressed as the means ± SD of three independent experiments. Statistical significance was determined by a Student’s t-test using GraphPad Prism. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001

Article Snippet: On day 35, dorsal telencephalic neurons were characterized with specific markers and used for ZIKV infection. hNPC line 15167 derived from fetal brains (Lonza) was kindly provided by S. Bao (Cleveland Clinic) 50 and cultured as neurospheres in neurobasal complete medium (Gibco) supplemented with B-27 supplement without Vitamin A (Thermo Fisher Scientific), L-glutamine (MACGENE, 2 mM), sodium pyruvate (MACGENE, 1 mM), basic fibroblast growth factor (bFGF, R&D Systems, 10 ng/mL), and epidermal growth factor (EGF, R&D Systems, 10 ng/mL).

Techniques: Infection, Control, Quantitative RT-PCR, Northern Blot, Stable Transfection